SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Kanawapee,Nantawan; Sanitchon,Jirawat; Srihaban,Pranee; Theerakulpisut,Piyada.

Thirty rice cultivars were evaluated for salinity tolerance during the seedling stage and were divided into five tolerance groups including tolerant (T), moderately tolerant (MT), moderately susceptible (MS), susceptible (S) and highly susceptible (HS) which comprised 5, 10, 9, 4 and 2 cultivars respectively. Genetic diversity of all rice cultivars was evaluated using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers. The cultivars were evaluated for polymorphisms after amplification with 20 random decamer primers and 20 SSR primer pairs. A total of 161 RAPD markers and 190 SSR alleles were produced which revealed 68.94% and 89.47% polymorphism respectively. Mean genetic similarity coefficient was 0.82 for RAPD and 0.70 for...

Tipo: Journal article

Palavras-chave: Genetic similarity; RAPD markers; Rice; Salinity tolerance; SSR markers.

Ano: 2011

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600002

Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)

Neeraja,Chirravuri Naga; Vemireddy,Lakshminarayana Reddy; Malathi,Surapaneni; Siddiq,Ebrahimali Abubacker.

After the success of IR8 and TN1, breeders depended heavily on these two rice cultivars for source of short stature led to the narrow genetic base to majority of present day rice varieties, as far as sd1 (semi-dwarf1) gene is concerned. In addition, analysis of genetic lineage of the majority of the cultivated rice varieties in tropical Asia reveals that sd1 from DGWG (Dee-Gee-Woo-Gen) is the major source of dwarfing gene. Such high amount of genetic homogeneity renders rice plants vulnerable to epidemic of diseases and insect pests. In the current study, we made an attempt to identify the alternate sources of DGWG allele of sd1 gene by characterizing 29 induced and 3 spontaneous dwarf accessions employing marker for DGWG allele of sd1 gene and exogenous...

Tipo: Journal article

Palavras-chave: Dee-Gee-Woo-Gen; Dwarfing gene; Gibberellic acid assay; Molecular marker; Rice; Sd1 gene.

Ano: 2009

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300007

Effects of 5-aminolevulinic acid (ALA)-containing supernatants from selected Rhodopseudomonas palustris strains on rice growth under NaCl stress, with mediating effects on chlorophyll, photosynthetic electron transport and antioxidative enzymes

Nunkaew,Tomorn; Kantachote,Duangporn; Kanzaki,Hiroshi; Nitoda,Teruhiko; Ritchie,Raymond J.

Background: Rice is globally one of the most important food crops, and NaCl stress is a key factor reducing rice yield. Amelioration of NaCl stress was assessed by determining the growth of rice seedlings treated with culture supernatants containing 5-aminolevulinic acid (ALA) secreted by strains of Rhodopseudomonas palustris (TN114 and PP803) and compared to the effects of synthetic ALA (positive control) and no ALA content (negative control). Results: The relative root growth of rice seedlings was determined under NaCl stress (50 mM NaCl), after 21 d of pretreatment. Pretreatments with 1 μM commercial ALA and 10X diluted culture supernatant of strain TN114 (2.57 μM ALA) gave significantly better growth than 10X diluted PP803 supernatant...

Tipo: Journal article

Palavras-chave: 5-Aminolevulinic acid (ALA); Antioxidative enzymes; Chlorophyll fluorescence; Rhodopseudomonas palustris; Rice; Salinity.

Ano: 2014

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100004

Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces

Pervaiz,Zahida Hassan; Rabbani,M. Ashiq; Khaliq,Ishtiaq; Pearce,Stephen R; Malik,Salman A.

Genetic diversity underlies the improvement of crops by plant breeding. Land races of rice (Oryza sativa L.) can contain some valuable alleles not common in modern germplasm. The aim here was to measure genetic diversity and its effect on agronomic traits among rice land-race genotypes grown in Pakistan. Diversity was measured using thirty-five microsatellite markers and seventy-five genotypes. Among the markers used a total of 142 alleles were detected at 32 polymorphic SSR loci, while three loci were monomorphic in Pakistani rice landraces. The number of alleles identified by each marker ranged from 2 to 13 with a mean of 4.4. Size differences between the smallest and largest alleles varied from 11bp to 71bp. Polymorphism information content ranged from...

Tipo: Journal article

Palavras-chave: Genetic diversity; Landraces; Microsatellite markers; Oryza sativa L.; Pakistan; Rice.

Ano: 2010

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300004

Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers

Ghneim Herrera,Thaura; Posso Duque,Duina; Pérez Almeida,Iris; Torrealba Núñez,Gelis; Pieters,Alejandro J; Martinez,César P; Tohme,Joe M.

In Venezuela, pedigree analyses indicate that the rice varieties currently under cultivation are closely related. Effective breeding programs, based on knowledge of the genetic diversity of cultivars, are needed to broaden the genetic bases of rice germplasm in the country. In this study, we used a set of 48 simple-sequence-repeat (SSR) markers to assess the genetic diversity of 11 Venezuelan rice cultivars, released by the National Rice Breeding Program between 1978 and 2007. A total of 203 alleles were detected, the number of alleles (NA) per marker ranged from 2 to 9, with an average of 4.23. The average genic diversity (H) over all SSR loci for the 18 genotypes was 0.524, ranging from 0.105 to 0.815. Positive correlations were found between H at each...

Tipo: Journal article

Palavras-chave: DNA fingerprinting; Genetic diversity; Rice; SSR.

Ano: 2008

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500003

Evaluation of genetic diversity and linkage disequilibrium in Korean-bred rice varieties using SSR markers

Iron homeostasis related genes in rice

Wang,Xiao-Qiang; Kwon,Soon-Wook; Park,Yong-Jin.

Background: In order to evaluate the variation among different rice types, the genetic diversity in a rice collection composed by 59 breeding lines, 23 landraces, 18 weedy rice lines, and 35 introduced lines that collected from countries worldwide was analyzed using 134 simple sequence repeat markers. Results: In total, 1264 alleles were identified (average, 9.43 per locus). Rare alleles made up a large portion (58.4%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbour-joining tree method were fairly consistent with the structure-based membership assignments for most of the...

Tipo: Journal article

Palavras-chave: Landraces; Linkage disequilibrium; Population structure; Rice; Simple sequence repeats.

Ano: 2013

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500011

Gross,Jeferson; Stein,Ricardo José; Fett-Neto,Arthur Germano; Fett,Janette Palma.

Iron is essential for plants. However, excess iron is toxic, leading to oxidative stress and decreased productivity. Therefore, plants must use finely tuned mechanisms to keep iron homeostasis in each of their organs, tissues, cells and organelles. A few of the genes involved in iron homeostasis in plants have been identified recently, and we used some of their protein sequences as queries to look for corresponding genes in the rice (Oryza sativa) genome. We have assigned possible functions to thirty-nine new rice genes. Together with four previously reported sequences, we analyzed a total of forty-three genes belonging to five known protein families: eighteen YS (Yellow Stripe), two FRO (Fe3+-chelate reductase oxidase), thirteen ZIP (Zinc regulated...

A walk on the wild side: Oryza species as source for rice abiotic stress tolerance

Palavras-chave: Iron; Rice; Genome analysis; Iron transporters; Ferritin.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400012

Menguer,Paloma Koprovski; Sperotto,Raul Antonio; Ricachenevsky,Felipe Klein.

Abstract Oryza sativa, the common cultivated rice, is one of the most important crops for human consumption, but production is increasingly threatened by abiotic stresses. Although many efforts have resulted in breeding rice cultivars that are relatively tolerant to their local environments, climate changes and population increase are expected to soon call for new, fast generation of stress tolerant rice germplasm, and current within-species rice diversity might not be enough to overcome such needs. The Oryza genus contains other 23 wild species, with only Oryza glaberrima being also domesticated. Rice domestication was performed with a narrow genetic diversity, and the other Oryza species are a virtually untapped genetic resource for rice stress tolerance...

Comparative linkage mapping of Oryza glumaepatula and Oryza sativa interspecific crosses based on microsatellite and expressed sequence tag markers

Palavras-chave: Oryza; Rice; Wild species; Abiotic stress; Domestication.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200238

Rangel,Priscila Nascimento; Brondani,Rosana Pereira Vianello; Coelho,Alexandre Siqueira Guedes; Rangel,Paulo Hideo Nakano; Brondani,Claudio.

Molecular linkage maps representing the rice genome have been an important tool for breeding programs because they allow the elucidation of polygenic traits and are an efficient tool for monitoring wild introgressions in interspecific crosses. Common markers among rice genetic maps are important in defining the homology of chromosomes and the synteny between genomic target regions. We used 148 markers (expressed sequence tags, microsatellites and single nucleotide polymorphisms) to construct a molecular linkage map based on co-dominant markers for an interspecific backcross population using a wild rice (Oryza glumaepatula) from Brazil and performed a comparative analysis with other interspecific maps. The comparative analysis revealed a Spearman...

DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars

Palavras-chave: Comparative linkage map; Molecular markers; Oryza glumaepatula; Rice.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400019

Sandhu,S.S.; Colombo,Carlos; Bastos,Cândido R.; Siqueira,Walter J..

Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate...

A new approach for obtaining rapid uniformity in rice (Oryza sativa L.) via a 3x x 2x cross

Palavras-chave: Rice; SCAR; Arbitrary primers; Pyricularia oryzae.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011

Xing,Shaochen; Cai,Yuhong; Zhou,Kaida.

A triploid (2n = 3x = 36) rice plant was obtained by screening a twin seedling population in which each seed germinated to two or three sprouts that were then crossed with diploid plants. One diploid plant was chosen among the various F1 progenies and developed into an F2 population via self-pollination. Compared with the control variety Shanyou 63, this F2 population had a stable agronomical performance in field trials, as confirmed by the F-test. The stability of the F2 population was further substantiated by molecular analysis with simple sequence repeat markers. Specifically, of 160 markers assayed, 37 (covering all 12 chromosomes) were polymorphic between the parental lines. Testing the F1 hybrid individually with these markers showed that each PCR...

Overexpression of salt-induced protein (salT) delays leaf senescence in rice

Palavras-chave: F-test; Polyploidy; Rice; SSR marker; Stability.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200020

Zhu,Keming; Tao,Huimin; Xu,Shuo; Li,Kaixia; Zafar,Sundus; Cao,Wei; Yang,Yanhua.

Abstract Senescence, a highly programmed process, largely determines yield and quality of crops. However, knowledge about the onset and progression of leaf senescence in crop plants is still limited. Here, we report that salt-induced protein (salT), a new gene, may be involved in leaf senescence. Overexpressing salT could prolong the duration of leaves with higher concentrations of chlorophyll compared with the wild type. Moreover, overexpression of salT could delay the senescence of rice leaves though the inhibition of senescence associated genes (SAGs). Overall, the characterization of salT suggested that it is a new gene affecting the leaf senescence induced by natural and dark conditions.

Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis

Palavras-chave: Leaf senescence; Salt-induced protein (salT); Rice; Stay-green.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100080

Hwang,Jung Eun; Hwang,Sun-Goo; Jung,In Jung; Han,Sung Min; Ahn,Joon-Woo; Kim,Jin-Baek.

Abstract Ionizing radiation has a substantial effect on physiological and biochemical processes in plants via induction of transcriptional changes and diverse genetic alterations. Previous microarray analysis showed that rice OsFBX322, which encodes a rice F-box protein, was downregulated in response to three types of ionizing radiation: gamma irradiation, ion beams, and cosmic rays. In order to characterize the radiation-responsive genes in rice, OsFBX322 was selected for further analysis. OsFBX322 expression patterns in response to radiation were confirmed using quantitative RT-PCR. Transient expression of a GFP-OsFBX322 fusion protein in tobacco leaf epidermis indicated that OsFBX322 is localized to the nucleus. To determine the effect of OsFBX322...

Genetic diversity analysis of Cuban traditional rice (Oryza sativa L.) varieties based on microsatellite markers

Palavras-chave: F-box protein; Gamma-irradiation; Ionizing radiation; Rice; Transgenic plant.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100304

Alvarez,Alba; Fuentes,Jorge Luis; Puldón,Violeta; Gómez,Pedro Julio; Mora,Leonor; Duque,Miriam C.; Gallego,Gerardo; Tohme,Joe M..

Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationship for breeding purposes. Heterozygosity was analyzed at each microsatellite loci and for each genotype using 10 microsatellite primer pairs. Between varieties genetic relationship was estimated. The number of alleles per microsatellite loci was 4 to 8, averaging 6.6 alleles per locus. Higher heterozygosity (H) was found in traditional varieties (H TV = 0.72) than in improved varieties (H IV = 0.42), and 68% of the total microsatellite alleles were found...

Estágios imaturos de Oebalus poecilus (Heteroptera, Pentatomidae)

Palavras-chave: Genetic diversity; Microsatellite; Rice; Traditional germplasm.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600014

Iheringia, Sér. Zool.

Greve,Caroline; Fortes,Nora D. F.; Grazia,Jocélia.

The immature stages of the rice bug Oebalus poecilus (Dallas, 1851) are described. The egg and the external morphological characters of the five nymphal stages were described and illustrated. The nymphs were exposed to two different photoperiods to induce seasonal forms to detect morphological differences.